In the course of a public appearance to a cathedral in Staffordshire on Monday, King Charles was confronted by a individual who asked him about Prince Andrew's links with disgraced financier Epstein.
The protester demanded to know how long the King had been known about his sibling's association with Jeffrey Epstein, and also questioned whether he had instructed the authorities to "conceal" for Andrew.
Despite the heckling, there were throngs of well-wishers and cheers for the monarch, who was visiting Staffordshire for an engagement.
Prince Andrew's connections with Epstein have been under renewed focus in recent weeks. The royal, who gave up his royal duties earlier this month, has strongly denied any wrongdoing.
"How long have you been aware about Prince Andrew and Epstein? Did you request the police to conceal for Andrew?"
The King remained silent but continued greeting supporters, some of whom chanted "God Save The King". One supporter can be overheard asking him for a embrace.
Another woman outside the venue was also heard telling the heckler to "leave" and to "be quiet".
The controversy over the Duke of York's connections to Epstein has been revived by sexual abuse claims repeated in Ms. Giuffre's recently released memoir.
In the publication, the accuser claims she had sex with the prince on three separate occasions, including one instance with Epstein and "eight other young girls"
Prince Andrew, who reached a financial settlement with the plaintiff in recent years, has consistently denied any wrongdoing. In 2019, he told a news program he did not remember meeting Ms Giuffre "ever", and that they "never had any sort of intimate relations."
But in recent times, fresh questions have been raised about how he is able to fund his way of life despite not being a working royal.
The revelation that he has only ever paid a nominal fee on his Royal Lodge has heightened the examination over his living arrangements - even though he made significant contributions initially, including for improvements.
The political party said he should be summoned front of MPs to give evidence about his rental agreement of his residence.
But last week, the authorities declined to give MPs time to debate the prince's titles or his home, despite growing pressure for scrutiny.
Buckingham Palace has not commented on whether the Duke of York might move out of his home - or where he might reside.
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