In the course of a public appearance to a cathedral in Staffordshire on the start of the week, the King was confronted by a protester who asked him about Prince Andrew's connections with convicted sex offender Jeffrey Epstein.
The individual asked how long the monarch had been known about his sibling's association with Epstein, and also questioned whether he had instructed the authorities to "conceal" for Prince Andrew.
Amid the heckling, there were throngs of supporters and cheers for the monarch, who was visiting the region for an event.
Prince Andrew's connections with Jeffrey Epstein have been under renewed focus in recent weeks. The royal, who gave up his titles earlier this month, has categorically denied any misconduct.
"How long have you known about Prince Andrew and Epstein? Did you request the authorities to conceal for Andrew?"
The monarch ignored the questions but continued meeting fans, some of whom chanted "Long live the King". One supporter can be overheard asking him for a embrace.
Another woman outside the cathedral was also heard telling the protester to "leave" and to "be quiet".
The scandal over Prince Andrew's connections to Epstein has been brought back into focus by misconduct allegations repeated in Ms. Giuffre's posthumously-published memoir.
In the memoir, Ms Giuffre says she had sex with the prince on three separate occasions, including one instance with Jeffrey Epstein and "several other minors"
Andrew, who reached a out-of-court agreement with Ms Giuffre in 2022, has repeatedly denied any wrongdoing. In 2019, he told a news program he did not remember meeting her "ever", and that they "never had any sort of sexual contact."
But in recent times, fresh questions have been raised about how he is able to fund his lifestyle despite not being a active member of the royal family.
The revelation that he has only ever paid a token annual rent on his Windsor mansion has intensified the examination over his housing situation - even though he made significant contributions initially, including for improvements.
The Liberal Democrats said he should be called in front of members of parliament to give evidence about his lease of his lodge.
But last week, the government declined to give MPs time to debate the royal's titles or his residence, despite growing pressure for scrutiny.
The royal household has not commented on whether the Duke of York might relocate of his home - or where he might reside.
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